c36950 mouse cxcl13 blc bca 1 quantikine elisa r d systems  (R&D Systems)

Journal: Journal of Hepatocellular Carcinoma

Article Title: Gut Microbiome and Hepatic Transcriptomic Determinants of HCC Development in Mice with Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JHC.S485532

Figure Lengend Snippet: Significant association between tumor burden and protein biomarkers CXCL13 and SCF. ( A ) Scatterplot showing correlation between tumor burden (mm 3 ) and serum CXCL13 protein concentration (ng/mL) in the Hep Pten − discovery female mice set. ( B ) Correlation between CXCL13 hepatic expression using RNA sequencing measured in adjusted counts per million (CPM) and serum CXCL13 protein concentration (ng/mL) from the Hep Pten − discovery female mice set. ( C ) Scatterplot showing correlation between tumor burden (mm 3 ) and SCF plasma protein concentration (pg/mL) in the Hep Pten − discovery female mice set. ( D ) Correlation between SCF hepatic expression using RNA sequencing measured in adjusted counts per million (CPM) and SCF plasma protein concentration (pg/mL) from the Hep Pten − discovery female mice set. ( E ) Correlation between tumor burden and serum CXCL13 protein concentration (ng/mL) in the Hep Pten − validation female mice set. ( F ) Correlation between tumor burden and SCF plasma protein concentration (pg/mL) in the Hep Pten − validation female mice set. Spearman correlation was used for all six plots. Spearman correlation p-values and rank correlation coefficients are shown. p<0.05 was considered significant.

Article Snippet: CXCL13 concentration was measured in serum samples using the Quantikine ELISA Mouse CXCL13/BLC/BCA-1 Immunoassay (catalog #MCX130, R&D Systems (bio-techne), Minneapolis) according to manufacturer’s instructions.

Techniques: Protein Concentration, Expressing, RNA Sequencing Assay